Available courses

Microscopy course impart an understanding of the theoretical background as well as practical skills in many applications from biology, medicine and materials science. The course is  a highly interactive learning experience, practical hands-on training on the most modern systems available.

  • Microscopy OLM
    • Nature of light
    • Optics and objective correction level
    • Kohler alignment
    • Focus and digital image collection
  • Sample Preparation
    • Trimming
    • Orientation determination
    • Fixation for OLM and TEM
    • Resin embeddment
  • Microtomy
    • Thick sectioning (0.5 – 1.5 um) for OLM
    • Chromatic staining for OLM
    • Thin section (70 – 40 nm) for TEM - using DiATOME Diamond Knives
    • Post staining for TEM contrast
  • Transmission Electron Microscope (TEM)
    • Electron optics
    • Aberrations and astigmatisms
    • Beam specimen interaction / image formation
    • Column alignment
    • Focus and astigmatism correction
    • Digital image collection
    • Artifact identification
  • Scanning Electron Microscope (SEM)
    • Chemical specimen processing
    • Critical Point Drying (CPD) and HMDS - using EMS 850 CPD
    • Physical specimen processing
    • Freeze Drying (FD) - using EMS 750 FD and EMS 150T sputter coater
    • SEM Instrument
    • Beam / specimen interaction theory
    • Instrument parameters ( kV, spot size/beam current, WD, tilt)
    • Signal/ Mode selection SE, BSE, VP, UVD
    • Focus and astigmatism adjustment